Synthetic feeder mimetics for pluripotent stem cell culture
Development of synthetic substrates is very important for clinical-grade feeder-free and xeno-free culture of pluripotent stem cells (PSC). We touched these issues last year. Today I’d like to share...
View ArticleEffects of media supplements on mesenchymal stromal cell culture
Mesenchymal stromal cells (MSC) is the most commonly used type of stem cells in clinical trials. Due to low number in freshly isolated mononuclear cells, MSC usually undergo ex vivo expansion. There...
View ArticleImpact of fetal bovine serum on toxicity of mesenchymal stromal cell...
The recent SafeCell study, published in PLoS ONE, analyzed the safety profile of mesenchymal stromal cells (MSC), administered systemically in patients. I blogged about it here. One of the most...
View ArticleNot Lost in Translation – How to get “clinical grade”
We’re staring a new series of posts “Not Lost in Translation“. In this series, we will try to bridge the translational gaps between scientific discovery in research labs and clinical cell applications...
View ArticleCell Culture Basics: Freezing and thawing cells
We continue our series Cell Culture Basics. Today I collected some good videos about cell freezing and thawing techniques. Freezing cells Very good protocol for freezing and thawing cells: Very good...
View ArticleNot Lost in Translation – Considerations for of translational research in...
About the author: Reinout Hesselink is an independent consultant to both cell therapy companies as well as academia. He has been active in the field of regenerative medicine development for over 10...
View ArticleGadgets review: Tools for 3D cell culture
Since the biggest shift in cell culture from 2D to 3D, there is a boom of tools providers on the market. If you’re doing cell culture and thinking about switching to 3D, you may want to know all...
View ArticleSynthetic surfaces for pluripotent stem cell culture
I’d like to share new and very interesting review, published in Cell Regeneration. The authors address the question of pluripotent stem cell interaction with surfaced used in cell culture via adhesion...
View ArticleImpact of antibiotics on function of human cells in culture
Recently, I came across very interesting study, which assessed impact of antibiotics on differentiation of human adipose-derived stem cells. Antibiotics is still commonly used ancillary material in...
View ArticleOptimization of serum-free media for MSC expansion
Everyone who is entering the field of clinical expansion of mesenchymal stromal cells (MSC) facing the problem: available reliable supply of serum for cell culture. As number of MSC clinical trials is...
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